Figure 4
From: Systematic reference sample generation for multiplexed serological assays

Correlation of the calculated sample pool MFI values and the measured pool MFI values.
While (A) the correlation between the sample pool and the predicted values (a weighted sum of single sample values) are high, the single samples (B–D) show weaker correlation with the pool. This data shows that the unique coverage characteristic of the serum pool is due to the combination of the three samples.