Figure 2

Characteristics of streptavidin-artificial receptors (SA-AR).

(A) Artificial receptors can be inserted in many different cell types. Cells were incubated with 2 μM SA-ARs for 2 h at 37°C. After adding SA-488, cells were imaged with bright field and fluorescence (488 ex) microscopy. (B) CEM cells were incubated with different concentrations from 31 nM to 5 μM SA-ARs for 2 h at 37°C, followed by addition of SA-488. SA-488 on the cell surface was analyzed by flow cytometry using channel 1 for SA-488. (C) CEM cells were incubated with 2 μM SA-AR for 2 h at 37°C, followed by washes and incubation at various times from 0 to 30 h before addition of SA-488. Following treatment, the cells were analyzed by flow cytometry. All histograms are ungated.