Figure 4
From: Modifying cellular properties using artificial aptamer-lipid receptors
Streptavidin-Modified Cells Bind Biotin to Make Cell Assemblies.
(A) Scheme showing the cellular assembly. (B) Top: CEM cells modified with streptavidin-488 via SA-AR incubated with TDO5 aptamer that binds Ramos cells and then either CEM (control, left) or Ramos (target, right). Bottom: Ramos cells modified with streptavidin-488 via SA-AR incubated with sgc8 aptamer that binds CEM and then either Ramos (control, left) or CEM (target, right). (C) SA-AR modified cells are collected with streptavidin coated magnetic beads. CEM cells modified with SA-AR or a non-specific PDGF-AR were collected with streptavidin coated DynaBeads. Cells were lysed and the lysates were probed by Western blot for β-Actin. (1) Unmodified cells. (2) TD05-biotin (3) Sgc8-biotin. (4) PDGF AR. (5) SA-AR. (6) Empty. (7) CEM lysate.
