Figure 5

Thrombin-Modified cells cause clotting.

500 K healthy CEM cells were washed and incubated for 2 h at 37°C in 50 μL media (Tubes 1&2), 5 μM T27-AR (Tubes 3–6), or PDGF-AR (Tube 7). After washing, cells were incubated with buffer (Tube 1), 500 nM thrombin (Tubes 2,3,7), 500 nM thrombin pre-incubated with 1 μM T-15 (Tube 4), 1 μM scrambled T-15 (Tube 5), or 200 nM BSA (Tube 6) for 30 min at RT. After 5 washes, fibrinogen was added to Tubes 2–7. Clots formed and were visualized by adding 50 μL trypan blue stain.