Figure 2
From: Development of novel DNA vaccine for VEGF in murine cancer model
Neutralizing activity of immunized serum in HUVECs.
(a) Western blot analysis of cell lysates from HUVECs stimulated for 10 min with mVEGF at 5 ng/mL in the presence of immunized serum or control serum for p-ERK and total ERK under the same experimental condition. (b) Effects of immunized serum on VEGF-induced tube formation of HUVECs. HUVECs were plated on matrigel-coated plates at density of 1 × 105 cells/well and incubated in the presence of control serum or immunized serum. After 7 hours, capillary network were photographed and quantified. Representative endothelial tubes were shown. Magnification: 50×. Data were means ± S.E.M. of triplicates.
