Figure 5
Maternal high-fat diet stimulates apoptosis in 63-week old prostate tissue of male offspring.
(a–f) TUNEL (green) of prostate acini from chow- (a, c and e) and HFD-exposed (b, d and f) offspring at 16 (a and b), 26 (c and d) and 63 (e and f) weeks of age. Nuclei are stained with TO-PRO-3 (blue). (g) Quantification of the percentage of TUNEL-positive cells per image. N ≥ 32 images from ≥7 mice, scale bars = 50 μm.
