Figure 1
From: A robust TALENs system for highly efficient mammalian genome editing
Our improved TALENs system.
(A) Schematic view of the TALEN structure with the DNA-binding modules highlighted. Each DNA-binding module consists of 34 amino acids, where the RVDs in the 12th and 13th amino acid positions of each repeat specify the DNA base being targeted according to the cipher NG = T, HD = C, NI = A and NN = G or A. (B) The TALENs system we have improved. A widely-used plasmid backbone, pcDNA3.1(−), was chosen for the TALEN vector. The red asterisks indicate where the BsaI sites were in the original pcDNA3.1(−) plasmid. They have been destroyed by mutagenesis. The original neomycin-resistant gene was replaced by a gene encoding EGFP or DsRed. The TALEN coding sequence and an adjacent upstream T7 promoter can be isolated by digesting the construct with SacI and PmeI (in red) as a template for in vitro transcription of TALEN mRNAs.
