Figure 2
From: A robust TALENs system for highly efficient mammalian genome editing
Enrichment of SKOV3 cells transfected with both TALEN constructs.
(A) Schematic view of TALENs that target human miR-210. The highlighted region indicates 22 nucleotides of mature miR-210 sequence. (B) Top panel, transfected SKOV3 cells before FACS. Approximately 50% and 20% of cells were transfected with a TALEN-L construct encoding EGFP and a TALEN-R construct encoding DsRed, respectively. Bottom panel, SKOV3 cells plated right after FACS. All cells are labelled by both EGFP and DsRed. Size of the bar, 100 μm. (C) Surveyor assay of DNA extracted from SKOV3 cells before and after FACS. The numbers at the bottom of the gel indicate mutation percentages measured by band intensities. Genomic editing is enhanced 3.8-fold following cell sorting.
