Figure 4
From: A robust TALENs system for highly efficient mammalian genome editing
In vitro translated TALEN proteins as an indicator for the quality of TALEN mRNAs.
The mRNAs encoding miR-210-targeting TALENs were transcribed in vitro. To check the quality of in vitro transcribed TALEN mRNAs, in vitro translation was performed and the translated proteins were separated on a 10% SDS-PAGE gel and detected by Western blotting using an anti-FLAG antibody. Proteins were successfully produced from both TALEN mRNAs, suggesting that they can be used for microinjection.
