Figure 2

Cellular response to TFIIB knockdown in human cells.

(A) TFIIB-siRNA knockdown in HeLa cells 90 h post transfection. TFIIB protein in cell lysate by Western blot is shown (lane 2); control siRNA (CsiRNA, lane 1), BsiRNA (lane 2) and transcription factor YY1siRNA (lane 3) are shown for comparison. Antibodies are indicated on the left and position of protein markers are on the right. TFIIB knockdown efficiency was also assessed by immunofluorescence: TFIIB (red) and DAPI (blue). The unprocessed image is shown in Supplemental Figure 6. (B) Apoptosis in cells with B-, YY1- and CsiRNA knockdown. The diagram presents the % of apoptotic cells from three independent experiments for the indicated cell types: C - control, B - BsiRNA, YY1 – YY1siRNA. The total amount of cells is taken as 100%; (C) Thymidine incorporation assay in siRNA-treated cells. Seventy hours after siRNA transfection, cells were given [³H]-thymidine for 12 hours: C-control, B- The data were calculated as the mean plus standard deviation from three experiments in triplicates BsiRNA, YY1 siRNA.