Figure 5

Antidepressant-like effect of inosine in CSDS test.

During 10 days of CSDS, mice were allowed access to water supplemented with (n = 10) or without inosine (1 mg/ml) (n = 12) ad libitum. Control mice (Ctl; n = 12) were individually housed without stress. (a–c) Food intake, liquid intake and body weight did not show any significant difference between stressed mice with and without inosine during CSDS test. (d) Inosine did not prevent aversive responses to aggressor mice. Social interaction was assessed by the following calculation: 100 × (interaction time, aggressor present)/(interaction time, aggressor absent). (e) Inosine prevented CSDS-induced reduction of preference for sucrose. Data represent mean ± s.e. *P < 0.05, **P < 0.01. (f–h) After CSDS, anxiety-like behavior and locomotive activity were evaluated by 10 min of OFT. Frequency of entry to the center in the arena by socially defeated mice tended to decrease compared with those of control mice, whereas inosine prevented this (f). However, there were no significant differences in distance traveled (g) and resting time (h) among the three groups. Data represent mean ± s.e.