Figure 2
Cellular accumulation and efflux of bodipy-FL-paclitaxel, a fluorescent ABCB1 substrate in lymphoblastoid and ovarian cancer cell lines measured by flow cytometry.
(a) Median bodipy-FL-paclitaxel accumulated in 13 LCLs and six ovarian cancer cell lines, by independent-samples median test; (b) Decreasing bodipy-FL-paclitaxel fluorescence intensity over a 10 minutes efflux time, in one LCL as a representative example; (c) A fitted curve generated by Solve function of Excel as compared to the actual curve of percentage fluorescence retained, in one LCL as a representative example; (d) Median efflux constant KE in LCLs and ovarian cancer cell lines, by independent-samples median test. All results are mean of at least two independent experiments.
