Figure 1
Tal2 was transiently expressed in P19 cells introduced into the neural differentiation and in embryogenesis.
P19 cells were treated with 1 μM atRA in suspension culture. (a) RT-PCR was performed to examine Tal2, Pou5f1 (the pluripotent transcription factor), Ascl1 (the neurogenic bHLH transcription factor) and Gapdh expression in P19 cells at 0, 3, 6, 12, 18, 24, 36 and 48 h. These images were cropped. Full-length gels are presented in Supplementary Figure S2. (b) Real-time PCR was performed to measure Tal2 expression at 0, 3, 6, 12 and 24 h. The gene expression levels of Tal2 were normalized using Hmbs gene as an internal standard. Fold expression was defined as fold increase relative to 0 h. Data represent the mean ± S.E. of three independent experiments. (c) RT-PCR was performed to examine Tal2 expression in mouse embryogenesis (E7, E11, E15 and E17) and adult brain. Tal2 expression was evident at E11. These images were cropped. Full-length gels are presented in Supplementary Figure S3.
