Figure 4
Tal2 expression was suppressed by RARα knockdown.
(a) The suppression of RARα in P19 cells was verified by Western blotting. The protein expression levels were normalized using β-actin as an internal standard and RARα expression was examined under the same conditions as β-actin. These images were cropped. Full-length gels are presented in Supplementary Figure S7. (b) Real-time PCR was performed to measure Tal2 expression in P19 cells subjected to RNAi-mediated RARα knockdown. A significant reduction of Tal2 expression was observed following RARα knockdown. Data represent the mean ± S.E. of three independent experiments. *, p < 0.05.
