Table 2 Quantitative parameters for analysis of AAs in brain tissue

From: Synergism and Rules of the new Combination drug Yiqijiedu Formulae (YQJD) on Ischemic Stroke based on amino acids (AAs) metabolism

Chemicals

Equationa

Correlation coefficient (r2)

Linear range (ng·mL−1)

LODb (ng·mL−1)

LOQc (ng·mL−1)

Ala

y = 3.0611x + 0.0165

0.9999

5–5000

0.53

1.79

GABA

y = 1.1833x − 0.0018

0.9991

5–5000

0.31

1.05

Ser

y = 1. 0241x + 0.0230

0.9997

5–5000

0.49

1.67

Asp

y = 0.6314x + 0.0040

0.9997

5–5000

0.49

1.67

Gly

y = 0.5793x + 0.0018

0.9990

5–5000

0.51

1.73

Hcy

y = 0.3371x + 07.73583 × 10−4

0.9966

1–200

0.10

0.35

Glu

y = 1.5217x + 0.0102

0.9999

5–5000

0.47

1.59

Met

y = 1.7855x + 0.0055

0.9993

1–500

0.05

0.18

Phe

y = 7.4569x + 0.0922

0.9981

1–500

0.02

0.07

Naa

y = 0.0789x − 2.50132 × 10−4

0.9994

5–5000

0.64

2.17

Tyr

y = 0.8392x + 0.0126

0.9981

1–500

0.05

0.18

Trp

y = 4.6319x + 0.0199

0.9996

1–500

0.13

0.43

  1. a. The calibration curves were constructed using relative responses versus the relative concentration of each analyte. Each calibration curve was derived from seven data points (n = 9), except Hcy (n = 7).
  2. b. LOD refers to the limit of detection in serum in ng·mL−1.
  3. c. LOQ refers to the limit of quantification in serum in ng·mL−1.
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