Table 1 Summary of experiments targeting the Sf-1 locus
From: Rapid generation of mouse models with defined point mutations by the CRISPR/Cas9 system
| Â | Microinjection | Survival of zygotes | Result of genotyping | Ratio of genotyping | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Experiment | gRNA | ssODN | 2 cell embryo/injected zygotes | Transferred | Genotyped | Monoallelic substitution (+indel) | Biallelic substitution (+indel) | Indel | WT | Mouse with substituted alleles (%) | Mouse with mutated alleles (%) |
1 | 1 | 1 | 128/256 | 128 | 39 | 0 (0) | 0 (0) | 31 | 8 | 0 (0) | 31 (79.5) |
2 | 1 | 2 | 101/203 | 101 | 37 | 1 (1) | 0 (0) | 32 | 4 | 1 (2.7) | 33 (89.2) |
3 | 2 | 3 | 124/169 | 124 | 51 | 8 (7) | 1 (1) | 26 | 16 | 9 (17.6) | 35 (68.6) |
4 | 3 | 1 | 157/217 | 144 | 54 | 10 (7) | 6 (5) | 28 | 10 | 16 (29.6) | 44 (81.5) |
