Table 3 Summary of off-target effects by SOX9-K396R gRNAs

From: Rapid generation of mouse models with defined point mutations by the CRISPR/Cas9 system

Target sites

Mutation rate with WT Cas9 (%)

Mutation rate with double nicking (%)

Sox9 locus

20/26 (76.9)

6/24 (25.0)

OT-1

0/26 (0)

0/24 (0)

OT-2

0/26 (0)

0/24 (0)

OT-3

0/26 (0)

0/24 (0)

OT-4

0/26 (0)

0/24 (0)

OT-5

0/26 (0)

0/24 (0)

  1. OT: Off-target site.
  2. Mutation rate: No. of mice with a mutated allele (including indels and substitutions)/No. of mice genotyped.
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