Figure 3

Eleven differentially expressed genes were selected for validation using qPCR.

Nine of the eleven genes exhibited average-fold changes in the same direction of change as qPCR, with SLC2A1 and RPS16 being the only exceptions. By comparison, a similar RNA-Seq validation using qPCR in the literature observed four out of five DE genes replicated³⁶. Additionally, correlation analysis (method described in Figure 2 from¹⁶) (Supplementary Table 1 and Supplementary Figure 2) generated average Pearson correlation coefficients of 0.57 and average Spearman correlation coefficients of 0.59 with an average best fit slope line of 0.87.