Figure 3

Experimental procedure and collected measures.

(A) Scheme of the three steps performed in this work for the injection of pairs of ablated clones. I) subclone preparation (from fragments of the same parental colony); II) tunic and vessels ablation in front of four to five zooids); III) parallel injection (one with the tested growth factor, the other with the solvent). (B) View of the five measures collected for each subclone (see Supplementary Table S1 for detailed information). Three measurements were collected the day of ablation, a few hours after the operation: i) colony size (dotted line); ii) ablated region size, calculated as the distance between lateral cut edges (polyline); iii) residual CCS area, delimited from the curved line in the ablated region. Two measurements were collected the following days: iv) the regenerated area after three days, delimited from the curved lines, which inner one delimitates the subtracted residual postoperative CCS area); v) days required for the regeneration of the marginal vessel (identified, in the ablated region, from the curved line). Arrowheads, marginal vessel stumps. Each step for the experimental procedure in (A) and for the measurements in (B) is flanked by a sketch. Scale bars: 1 mm. Images were organized with CorelDRAW X4 (Corel Corporation). Sketches were prepared with CorelDRAW X4 (Corel Corporation).