Figure 4 | Scientific Reports

Figure 4

From: Testing an unusual in vivo vessel network model: a method to study angiogenesis in the colonial tunicate Botryllus schlosseri

Figure 4

Immunostainig with antibodies against vertebrate angiogenic factor receptors.

Selected sections representative of B. schlosseri regenerating CCS in colonies injected with PBS. (A–C) The antibody antigen is EGFR. In (A) the dotted line indicates the cut edge of an operated colony two hours after the injection of PBS (approximately four hours after ablation of the CCS); (B) represents the boxed area in (A); the section in (C) is referred to an operated colony three days after ablation. (D, E) The antibody antigen is VEGFR-1; regenerating regions of operated colonies three days after ablation. (F) The antibody antigen is VEGFR-2; regenerating region of an operated colonies one day after ablation. Black arrowheads, stained apexes of elongating vessels (vl); black arrow, stained vessel stump after ablation; white arrows, stained apexes of ampullae (am); white arrowheads, stained sprouts. t, tunic Scale bars 150 μm in (A, C and D); 50 μm in (B, E and F); 100 μm in (C).

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