Figure 7
Confocal brightfield (a–d) and fluorescence (e–h) micrographs of rhesus embryos resulting from ICSI fertilization with oxidatively stressed sperm.
In each represented embryo, note extensive nuclear fragmentation and unequal blastomere size and integrity. Note abnormal mitotic figure in one embryo (g). Images were acquired using a 40×/NA 1.0 lens on an Olympus FV1000 Spectral Scan point scanning confocal running Olympus FluoView version 2.1 software. Noise in individual optical sections was reduced by summing multiple scans of each image (“Kalman filtering”). Differential Interference Contrast (DIC) images were recorded using unabsorbed excitation light at 488 nm. Three dimensional images were recorded after acquiring z-stack images of 1.62 um per sequential focal slice. Bar = 20μ.
