Figure 4
From: Production of salidroside in metabolically engineered Escherichia coli
Salidroside (1) and icariside D2 (4) were produced by metabolically engineered strain carrying Rhodiola-derived glycosyltransferase.
(a) HPLC analysis of tyrosol (3) and glycosylated derivatives in fermented supernatant sample of strains. 1. BMGA6 derived from BMGA5 by introduction of glycosyltransferase UGT73B6; 2. BMGA5 was a tyrosol over-producing strain; 3. Tyrosol (3) and salidroside (1) standard. (b) HR-ESI-MS of salidroside (3) from fermented supernatant of BMGA6. (c) Gastrodin (7), a main bioactive component from Tianma and its homolog icariside D2 (4). (d) HR-ESI-MS of icariside D2 (4) from fermented supernatant of strain BMGA6.
