Figure 1 | Scientific Reports

Figure 1

From: A human CCT5 gene mutation causing distal neuropathy impairs hexadecamer assembly in an archaeal model

Figure 1

The N-terminal portion of the amino-acid sequence alignment of human CCT5 (uniprot ID; P48643) and Pyrococcus furiosus PfCPN (uniprot ID; Q8TZL6) (A) with a 22-amino acids deletion in C-terminus, named PfCD (see B).

For a complete alignment see Supplementary Fig. S1. The residues conserved between human CCT5 and PfCD are shown in the space between their sequences. The secondary structures were predicted using the GOR IV server (http://npsa-pbil.ibcp.fr/cgi-bin/npsa_automat.pl?page=npsa_gor4.html) and α-helix, β-sheet and loop elements are represented as red rings, blue wave and black line, respectively, above or below their amino-acid sequences. The amino acids matching the human pathogenic mutation in CCT5 (His147) and the equivalent locus in the archaeal PfCD (Ile138) that we mutated to Ile138His and Ile138Arg - the humanized archaeal molecules - are shown in red bold letters and indicated with an asterisk.

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