Figure 2
(A). Human CCT5 and P. furiosus PfCD superposed onto the crystal structure of Thermococcus strain KS-1 α. LEFT: The structure (Swiss-Model (http://swissmodel.expasy.org/) of PfCD (gold) superposed onto the crystal structure of KS1α (momomers, displayed in marine blue, violet and deep teal as surface and in cyan color as ribbon). The hexadecamer, double-ring structure, is depicted by a dotted line, as is on the right of the subsequent panels B and C. RIGHT: Magnified image of the superposed structures of the PfCD (gold) and Human CCT5 (orange) onto KS1α; cyan ribbon). Side chains of isoleucine at 138 of PfCD (blue) and isoleucine at 138 of KS1α (deep teal) and side chain of histidine at 147 of human CCT5 (red) are represented as ball and stick. AMP-PNP (green stick; also shown on the left in panels B and C) and magnesium ion (yellow ball). (B). PfCD around the Arg138 mutation and its predicted matching areas in the crystal structure of KS1α. LEFT: Mutant Arg138 (orange) and its adjacent Arg421 (red) in the opposite α helix are drawn with stick in the PfCD structure (marine blue). RIGHT: Arg138 mutation area was superposed onto the crystal structure of KS1α (monomers are displayed in various shades of blue). (C). Human CCT5 structure around the Arg147 mutation viewed from different angles and predicted matching areas in the crystal structure of KS1α. LEFT: Wild-type His147 (blue), mutant Arg147 (red) and their adjacent amino acids, Ser428 and Cys429 (magenta) in the opposite α helix are drawn with ball and stick and transparent surface in the human CCT5 structure (orange ribbon). RIGHT: The predicted Arg147 mutation area in the monomers is shown as a red surface on the crystal structure of KS1α (monomers are shown as surface in marine blue, violet, deep teal and cyan colors.
