Figure 4
Oligomeric states of purified PfCD, I138H and I138R Cpns determined by gel filtration chromatography (top panel) and corresponding SDS-PAGE analysis (bottom panel).
PfCD, or I138H, or I138R purified by gel filtration chromatography followed by anion exchange chromatography were pooled together for ATPase activity and MDH heat-protection activity assays. The same amounts of these pooled pure Cpn proteins were subjected to 4–9% gradient native-PAGE analysis (top panel) and 12% SDS-PAGE analysis (bottom panel) and then stained with Coomassie brilliant blue G-250. The figures to the right, 16 on top and 1 at the bottom, indicate hexadecamer and monomer, respectively, while the other oligomers are distributed in between them as shown by the gel bands. The thick arrow at the very bottom indicates the monomeric band of the purified Cpns run with same amount of protein in SDS-PAGE. St, molecular weight standard.
