Table 1 Oligonucleotide primers used for RT-PCR and RT-qPCR

617.TcRyRF1

AGAATGGCGGAGGCCGAAG

RyR RT-PCR product P1(1-1208)

618.TcRyRR1

ACTCTCGCAGTTCTGGATTC

619.TcRyRF2

GACTTCAGTAGGAGTCAAGA

RyR RT-PCR product P2(1165-2569)

620.TcRyRR2

TGGAAGTGTCTACAGGGTTT

621.TcRyRF3

GAAAGCCTCCTCCCGCAACA

RyR RT-PCR product P3(2434-2855)

622.TcRyRR3

ATTCGCCTTCTCCATAGTCT

623.TcRyRF4

GGTTCAGACAGTCCTCCGTG

RyR RT-PCR product P4(2790-5356)

624.TcRyRR4

AGGCGGCATAAAGAGTCAAA

625.TcRyRF5

CGCTGTATTGATGTATTGGA

RyR RT-PCR product P5(5275-6654)

626.TcRyRR5

CCACATTTCGGCAACATCTT

627.TcRyRF6

GTACAATTTCATAAACGCCG

RyR RT-PCR product P6(6381-8034)

628.TcRyRR6

TCGGTAGACTGTGTGTAAAG

629.TcRyRF7

CACTCCTCATCCAACACAGC

RyR RT-PCR product P7(7949-9416)

630.TcRyRR7

CAAAACAGGGAAGCCACCAT

656.TcRyRF12

TTCATCCACCTTCAGTCGCA

RyR RT-PCR product P8(9222-11018)

657.TcRyRR12

TGCCAAGACATTTCGTCAGC

633.TcRyRF9

GTTTTGATAATGCCCACAGC

RyR RT-PCR product P9(10702-12302)

634.TcRyRR9

AAACCTCCAACAGCGTCCCA

635.TcRyRF10

GCTATCGGTGTTGCTAGTCA

RyR RT-PCR product P10(12187-13847)

636.TcRyRR10

GCACGATGACTGTAAGCACC

637.TcRyRF11

AACCTGTTGTTACTGAACCT

RyR RT-PCR product P11(13784-15115)

638.TcRyRR11

AGTTGTGCTCTTGTTGGACG

682.TcRyRF13

CATCGTTATTCTTCTGGCTA

RyR RT-PCR product P12(14934-15308)

683.TcRyRR13

CTGAAAGTGAATAGGAAGTG

734.TcIP₃RF1

CTGAAAACGCTCCAAAAACC

IP₃R RT-PCR product P1(1-1647)

735.TcIP₃RR1

CGTGTCTTGGGTCGTTCAGT

736.TcIP₃RF2

TTTGGACAACAACGGGGACG

IP₃R RT-PCR product P2(1586-3158)

737.TcIP₃RR2

AAAAATGCCCTCAGCTTGAC

738.TcIP₃RF3

TACCCGCTCGTCATGGATAC

IP₃R RT-PCR product P3(2949-4296)

739.TcIP₃RR3

ATGGCAGTAAACTATGACAC

740.TcIP₃RF4

AAGACACTGTATGGACGAGG

IP₃R RT-PCR product P4(4175-5698)

741.TcIP₃RR4

TCTTCTCTTAACTCGTCACT

742.TcIP₃RF5

CAAACAAGACGGGAAAGATT

IP₃R RT-PCR product P5(5609-6999)

743.TcIP₃RR5

TCCGTATGCCTGTCTCCCTA

744.TcIP₃RF6

CTCTTCTGGGTTAGTAGTTA

IP₃R RT-PCR product P6(6795-8231)

745.TcIP₃RR6

GACTAGGACAAGTTATCAAC

800.Tcrps3F1

ACCGTCGTATTCGTGAATTGAC

RT-qPCR

801.Tcrps3R1

ACCTCGATACACCATAGCAAGC

806.TcRyRF

AAGGGGTATCCTGATTTGGG

RT-qPCR (7198-7400)

807.TcRyRR

TTCGCATCTACGATAGCACG

808.TcIP₃RF

GTGACTTGAGCCAGGCTTTC

RT-qPCR (5491-5621)

809.TcIP₃RR

CCCGTCTTGTTTGTCCTCAT