Scientific Reports

Table 1 Amplification Efficiency of Short and Long Amplicons in Frozen and FFPE tissues

From: Quantitative assessment of short amplicons in FFPE-derived long-chain RNA

	Frozen tissue				FFPE tissue
RNA	Efficiency (%)		Coefficient (R²)		Efficiency (%)		Coefficient (R²)
	Short-A	Long	Short-A	Long	Short-A	Long	Short-A	Long
Endogenous control
β-actin	100	129	0.999	0.907	101	204	1.000	0.775
Target RNA
DD3	107	169	0.973	0.904	109	111	0.962	0.631
H19	105	163	0.999	0.852	106	2840	0.993	0.208
MALAT1	106	198	0.999	0.731	105	371	1.000	0.414
MEG3	100	135	0.988	0.892	111	618	0.983	0.521
p15AS	139	174	0.966	0.761	114	287	0.699	0.826
UCA1	158	249	0.985	0.877	271	-98	0.969	0.207
CDK6	106	221	0.988	0.798	96	-62	0.972	0.656
JUN	108	118	0.999	0.952	106	315	0.998	0.636
MAPK3	98	87	0.999	0.981	99	168	0.968	0.887
PDGFRA	107	141	0.999	0.897	102	525	0.996	0.46
PDGFRB	108	117	0.999	0.979	118	222	0.985	0.844
PIK3R3	103	215	0.962	0.782	110	-98	0.926	0.048
SMAD3	113	115	0.994	0.976	110	154	0.978	0.700
SMAD4	101	109	0.994	0.984	107	202	0.935	0.845
Mean ± SD	111 ± 0.2	156 ± 0.5	0.990 ± 0.0	0.885 ± 0.1	118 ± 0.4	384 ± 7.1	0.957 ± 0.1	0.577 ± 0.3
P value	0.0006		< 0.0001		0.0152		< 0.0001

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