Figure 1 | Scientific Reports

Figure 1

From: A multicolor panel of TALE-KRAB based transcriptional repressor vectors enabling knockdown of multiple gene targets

Figure 1

Construction of a multicolor panel of lentiviral vectors expressing TALE-KRAB.

(A) Maps of TALE-KRAB lentiviral vectors containing a multicolor panel of fluorescent proteins. TALE is fused to the N-terminus of the KRAB transcriptional repressor domain. Individual fluorescent protein gene is fused in-frame with KRAB via T2A sequence. Drug resistant genes Blasti and Zeo are at the downstream of mCherry and Venus fluorescent protein genes and separated by an IRES sequence. EF1a promoter drives TALE-KRAB, fluorescent protein genes and drug resistant genes. Blasti, resistance to Blasticidin S; Zeo, resistance to Zeocin. IRES, an internal ribosome entry site. NLS, nuclear localization signal. (B) Fluorescent images of 293T cells transfected with individual TALE-KRAB expression vectors. 293T cells were transfected with indicated vectors and fluorescent images were taken using an Olympus IX71 equipped with a color camera at 24 hrs after transfection.

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