Figure 3
Virus growth and innate immune response against DENV and JEV infection.
HeLa cells were infected with DENV2 strain 16681 at MOI 1 or 10 or JEV strain JaOArS982 at MOI 1. At the indicated time points, the culture fluid and the cells were harvested. (A) The virus titers in the culture fluid were determined by FFA. (B) The vRNA and the IFN-β gene expression in the DENV- and the JEV-infected cells that were quantified by RT-qPCR. The absolute vRNA copy numbers and the relative-fold increase in the IFN-β mRNA levels were normalized with GAPDH mRNA levels. The mean ± SE of the vRNA copy number was obtained from three independent duplicate experiments. The values between the DENV- (MOI 1) and JEV- (MOI 1) infected cells or the DENV- (MOI 10) and the JEV- (MOI 1) infected cells were tested by Welch test analysis. The single asterisks indicate a significant difference of vRNA (* p < 0.01) and double asterisks indicate a significant difference of IFN-β mRNA (** p < 0.01). (C) Representative cropped blots of RIG-I and GAPDH in DENV- and JEV-infected cells are shown. The infected cells were harvested and the target proteins were detected by the immunoblotting assay. GAPDH was used as an internal control. All the samples were derived from the same experiment and blotting was processed in parallel. The full-length blotting images are presented in Supplemental Fig. S4.
