Figure 4
Effect of viral dsRNA on the IFN activation pathway.
(A) One microgram/well of the mock RNA, the DENV RNA, or the JEV RNA was transfected into uninfected cells. (B) Five hundred nanogram/well of the non-treated, RNase R-treated or the RNase III-treated DENV RNA was transfected into uninfected cells. (C) The cells were infected with DENV2 strain 16681 at MOI 1 or 10 prior to the IFN-β stimulating assay. After 48 h p.i., 500 ng/well of the mock RNA or the DENV RNA were transfected into the cells with the uninfected control. Six hours after the RNA transfection, the cells were harvested and the IFN-β and the GAPDH mRNA levels were quantified by RT-qPCR. The relative-fold increase of the IFN-β mRNA levels was normalized with the GAPDH mRNA levels. The mean ± SE of the IFN-β mRNA levels were obtained from two independent duplicate experiments. The values between two groups were tested by Welch test analysis. The asterisks indicate statistical significance at p < 0.01 and ND indicate no significant difference.
