Figure 6
Immunofluorescence detection of the cytosolic DENV and JEV dsRNA.
(A) The localization of the dsRNA in DENV2 strain 16681 (MOI 1) and JEV strain JaOArS982 (MOI 1) infected cells were observed at 24 h p.i. The infected cells were permeabilized with 1% NP-40 or 0.5 mM of digitonin and stained for dsRNA using the immunofluorescence assay. The white arrowheads indicate the viral dsRNA. (B) The percentage of cells positively stained for dsRNA with 1% NP-40 or 0.5 mM digitonin permeabilization was calculated in 3 different fields (241.1 ± 10.8 total cells/field for NP-40 and 1241.4 ± 37.4 total cells/field for digitonin permeabilization). The percentages of positively stained cells were arcsine transformed prior to the Welch test analysis. The statistically significant differences between the DENV- and JEV-infected cells are indicated (* p < 0.01). The error bars indicate standard error of the means. The experiment was continued until the cells started dying (72 h p.i. for DENV and 48 h p.i. for JEV).
