Figure 3 | Scientific Reports

Figure 3

From: Over expression of hRad9 protein correlates with reduced chemosensitivity in breast cancer with administration of neoadjuvant chemotherapy

Figure 3

hRad9 is deregulated in MCF-7 and MDA-MB-231 breast cancer cells after hRad9-siRNA treatment.

Relative expression of hRad9 mRNA in MCF-7 and MDA-MB-231 breast cancer cells (a). RNA was isolated from cultures after 48 h in vitro growth and analyzed by RT-qPCR. Internal RT-qPCR control was GAPDH. Results are expressed as fold content in respect to MCF-7 cells (average ± SD; n = 3). Specific knockdown of hRad9 mRNA expression measured by RT-qPCR in MCF-7 and MDA-MB-231 breast cell lines after hRad9-siRNA in comparison to controls (b). The ΔΔCt method and normalization by comparison with controls were used. Values are means ± SD for n = 3 assays. Statistical analysis was carried out by Student t-test (P < 0.05). Western blot analysis confirmed the siRNA knockdown of hRad9. The fractionated cell extracts were analyzed with indicated antibodies by immunoblotting (c). hRad9 and chk1 protein expression were deregulated by siRNA knockdown of hRad9 compared to the scrambled siRNA or blank controls in breast cancer cell lines.

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