Figure 3
From: Orientation in multi-layer chitosan hydrogel: morphology, mechanism and design principle

Morphology of hydrogel prepared by single-opening mold.
(a)–(f) Micrographs of hydrogels: (a) CLSM image, c(CS) = 0.1 wt.%, cross section; (b) SEM image, c(CS) = 0.5 wt.%, longitudinal section; (c) CLSM images, c(CS) = 4.0 wt.%, longitudinal section; (d) SEM images, c(CS) = 3.0 wt.%, longitudinal section; (e) and (f) Fluorescence microscope images, bright field, colored by rhodamine for visibility, cross section and longitudinal section, respectively, c(CS) = 3.0 wt.%; (g) Schematic diagram of longitudinal and cross section of hydrogel, the longitudinal section is perpendicular to the primary hydrogel layer, while the cross section is parallel to it.