Figure 2 | Scientific Reports

Figure 2

From: Transcriptomic analysis reveals the roles of microtubule-related genes and transcription factors in fruit length regulation in cucumber (Cucumis sativus L.)

Figure 2

Verification of differentially expressed genes by qRT-PCR.

Fourteen DEGs with higher expression and six DEGs with lower expression in line 408 were chosen for qRT-PCR validation. The relative expression level of each gene was expressed as the fold change between two lines in the RNA-Seq data (white bar) and qRT-PCR data (gray bar). The cucumber UBIQUITIN gene was used as an internal control to normalize the expression data. The bars represent the standard deviation (n = 3). Asterisks indicate that the gene transcriptions are significantly different between lines 408 and 409 (unpaired t test, P < 0.05).

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