Figure 1
Leg phenotypes obtained by rdRNAi against Gb'dac and Gb'Dll during regeneration.
(a) DsRed2 rdRNAi control adult cricket with a normally regenerated right metathoracic (T3) leg. (b) Control regenerating leg of a fifth-instar nymph. (c) Higher magnification of panel b showing a control-regenerating tarsus. (d) Control regenerated leg of an adult. Normal tarsus consists of tarsal segments (Ta) 1, 2 and 3 and spurs and claws. (e) Gb'dac rdRNAi adult with a short regenerated T3 leg. (f) Regenerating leg of a fifth-instar nymph treated with Gb'dac rdRNAi. (g) Higher magnification of panel f showing a tarsus of Gb'dac rdRNAi regenerating leg. (h) Regenerated leg of a Gb'dac rdRNAi adult showing a normal Ta2 and 3 and claws but lacking Ta1 and having a short tibia. (i) Gb'Dll rdRNAi adult (injection of 20 μM Gb'Dll dsRNA). (j) Regenerating leg of a fifth-instar nymph treated with Gb'Dll (20 μM) rdRNAi. (k) Tarsus of regenerating Gb'Dll (20 μM) rdRNAi leg at higher magnification than shown in j. (l) Regenerated leg of a Gb'Dll (20 μM) rdRNAi adult. Gb'Dll (20 μM) rdRNAi caused extreme reduction of all tarsal structures. (m) A Gb'Dll (0.2 μM) rdRNAi adult. (n) Regenerating leg of a fifth-instar nymph treated with Gb'Dll (0.2 μM) rdRNAi. (o) Higher magnification of panel n showing a tarsus of Gb'Dll (0.2 μM) rdRNAi regenerating leg. (p) Regenerated leg of a Gb'Dll (0.2 μM) rdRNAi adult. A long Ta1 was formed. (b–c, f–g, j–k and n–o) Scanning electron microscopy (SEM) of rdRNAi regenerating legs. Red arrowheads in (a–b), (e–f), (i–j) and (m–n) indicate the amputation site. Arrows indicate spurs of the tibiae in (c), (d), (g) and (h). Arrowheads indicate spurs of the tarsi in d and h. Scale bars; 5 mm in (a) and (d). Ta1–3, tarsal segments 1–3; Ti, tibia; Cl, claws.
