Figure 2
Appearance of phosphoethanolamine-enriched PFCE nanoparticles.
(a) Cryogenic transmission electron microscopy (TEM) images of DSPE-PEG2000, Pluronic-basic, Pluronic-DPPE and Pluronic-DPPE-Rhodamine nanoparticles encapsulating PFCE fluorine compound (size-bar for TEM images: 50 nm). (b) Upper two panels and lower left panel show ultrathin sections of DCs labeled with DSPE-PEG2000, basic and DPPE-enriched PFCE nanoparticles (size-bar for EM images: 2 μm). Lower right panel shows a laser scanning microscopy image of DCs labeled with DPPE-Rhodamine-enriched 19F nanoparticles (size-bar for LSM image: 10 μm). (c) DCs were labeled with different nanoparticle preparations (DSPE-PEG2000 NP, basic NP, DPPE-NP and DPPE-Rhodamine-NP) using a PFCE concentration of 10 μmol per 107, fixed in 2% PFA and transferred (106) to NMR tubes. 19F signal was acquired using a 90° block excitation pulse and the PFCE amount per 106 calculated using a 500 mM PFCE standard.
