Figure 2
Nuclear lobulation of YB-1 KD cells.
(A, B) At 48 h post transfection with either non-targeting (control) or YB-1 siRNA (YB-1 KD), HeLa-EB1-GFP cells were stained with DAPI. The arrowheads indicate EB1-GFP accumulating in the centrosome. Dotted lines indicate the outline of each cell. Scale bar, 5 μm. The ratio of lobulated nuclei to the total cell number is indicated in Fig. 2B. The data represent mean values with standard deviations from three independent experiments (n > 250). (C) At 48 h post transfection with either non-targeting (control) or YB-1 siRNA (YB-1 KD), HeLa cells were subjected to the indirect immunofluorescence assay with anti-lamin A/C (green) and NUP214 (red) antibodies, respectively. The annulate lamellae and the discontinuous regions of nuclear lamina are indicated by arrows and arrowheads, respectively. Scale bar, 5 μm. (D) At 48 h post transfection with either non-targeting (control) or YB-1 siRNA (YB-1 KD), HeLa cells were subjected to transmission electron microscopic analysis. Nuclear envelope is indicated by arrowheads.
