Figure 4 | Scientific Reports

Figure 4

From: Centrosome maturation requires YB-1 to regulate dynamic instability of microtubules for nucleus reassembly

Figure 4The alternative text for this image may have been generated using AI.

Low Concentration of Nocodazole Attenuates the Formation of Lobulated Nuclei in YB-1 KD Cells.

At 12 h post transfection with either non-targeting (control) or YB-1 siRNA (YB-1 KD), the cells were treated with either 3 nM, 10 nM nocodazole, or 2 mM thymidine for 36 h. (A) At 48 h post transfection, the number of lobulated nuclei was counted. The data represent mean values with standard deviations from three independent experiments (n > 250). (B) The number of cells was determined in the absence or presence of 10 nM nocodazole at 24 and 48 h post transfection, respectively. (C) Representative results summarized in Fig. 4A are shown. Nuclei were stained with DAPI. Dotted lines indicate the outline of each cell. The lobulated nuclei or micro nuclei are indicated by arrowheads. Scale bar, 50 μm.

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