Figure 2
From: Production of diacetyl by metabolically engineered Enterobacter cloacae
Molecular authentication and metabolic production analysis of E. cloacae SDM and its derivatives.
Panel I: E. cloacae SDM; Panel II: SDM (ΔbudA); Panel III: SDM (ΔbudAΔgdh); Panel IV: SDM (ΔbudAΔbudC). (A): Analysis of PCR fragments to confirm disruption of the gene. Lane M, molecular mass standard (Trans5K). (B): Fermentation products identified by GC. (C): Colorimetric detection of diacetyl (1, 2, 3 means all assays were performed by triplicate cultures). I-A: Lane 1–3: budA, gdh, budC products amplified with SDM genomic DNAs as the template. II-A: Lane 1–2, budA products amplified with SDM and SDM (ΔbudA) genomic DNAs as the templates, respectively. III-A: lane 1–2: gdh products amplified with SDM (ΔbudAΔgdh) and SDM genomic DNAs as the templates, respectively; lane 3–4: budA products amplified with SDM (ΔbudAΔgdh) and SDM genomic DNAs as the templates, respectively. IV-A: Lane 1–2: budC products amplified with SDM (ΔbudAΔbudC) and SDM genomic DNAs as the templates, respectively; lane 3–4: budA products amplified with SDM (ΔbudAΔbudC) and SDM genomic DNAs as the templates, respectively.
