Figure 5
From: Production of diacetyl by metabolically engineered Enterobacter cloacae
Time course of batch fermentation of diacetyl under the optimization condition.
Glucose (
), Cell density (
), α-Acetolactate (
), Diacetyl (
). (A) Time course of diacetyl and α-acetolactate production using E. cloacae SDM (ΔbudAΔbudC) before Fe3+ addition. (B) Time course of diacetyl production after 20 mM Fe3+ added at 10 h. The bioconversion was carried out at 37°C in 300-mL shake flasks containing 50 mL of medium with pH adjusted to 7.4. The initial glucose concentration used was 17.8 g/L. Error bars indicate standard deviation (n = 3).
