Table 3 Bacterial strain and plasmid used in this studya
From: Production of diacetyl by metabolically engineered Enterobacter cloacae
Strain or plasmid | Characteristic(s) | Source or reference |
|---|---|---|
Strain | ||
E. coli DH5α | F−, φ80 lacZΔM15, Δ(lacZYA-argF)U169, recA1, endA1, hsdR17, phoA, supE44λ−, thi−1, gyrA96, relA1 | Novagen |
E. coli S17-1 | recA, pro, thi, conjugative strain able to host λ-pir-dependent plasmids | |
E. cloacae SDM | Wild-type | |
SDM (ΔbudA) | E. cloacae SDM budA disruption mutant strain | This study |
SDM (ΔbudAΔbudC) | E. cloacae SDM budA and budC disruption mutant strain | This study |
SDM (ΔbudAΔgdh) | E. cloacae SDM budA and gdh disruption mutant strain | This study |
SDM (ΔbudAΔbudCΔgdh) | E. cloacae SDM budA, budC and gdh disruption mutant strain | This study |
Plasmid | ||
pEASYBlunt | Apr, cloning vector | Transgen |
pKR6K | Kmr, gene replacement vector derived from plasmid pK18mobsacB, R6K origin, Mob+ sacB | |
pKΔbudA | Kmr, pKR6K derivative, carries a 587 bp deletion of budA | This study |
pKΔbudC | Kmr, pKR6K derivative, carries a 639 bp deletion of budC | This study |
pKΔgdh | Kmr, pKR6K derivative, carries a 302 bp deletion of gdh | This study |
