Figure 2 | Scientific Reports

Figure 2

From: ZNF32 inhibits autophagy through the mTOR pathway and protects MCF-7 cells from stimulus-induced cell death

Figure 2

ZNF32 inhibits autophagy initiation by activating the AKT/mTOR pathway.

(A) Effects of ZNF32-KD or ZNF32-OE on p27, Atg5, Beclin-1, p-AKT and p-mTOR expression. (B) mTOR activation in MCF-7 cells with ZNF32 overexpression after a 24 h treatment with 100 nmol/L rapa was determined using western blot analysis. (C) Phosphorylation of mTOR and AKT in MCF-7 cells with ZNF32 overexpression or knockdown after a 24 h treatment with 1 ng/ml EGF. (D) AO staining in MCF-7 ZNF32-KD or ZNF32-OE cells after 24 h treatment with 100 nmol/L rapa or 1 ng/ml EGF was detected using fluorescence microscopy. (E) ZNF32 expression rescues AKT/mTOR activation and LC3 II expression in ZNF32 knockdown cells. β-actin was used as a loading control. The full size blots were shown in the Supplementary Figure S5 and band of interest is indicated with an arrow.

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