Figure 3
ZNF32-associated autophagy participates in H2O2-and diamide- induced cell death.
(A) MTT assay showing the effect of ZNF32 on cell viability after 24 h treatment with H2O2 (700 μmol/L) or diamide (500 μmol/L). (B) LC3 II expression after a 24 h treatment with H2O2 or diamide in ZNF32-KD cells. (C) Images of AO staining in ZNF32-KD cells after a 24 h treatment with H2O2 or diamide with a 3 h pre-treatment with or without Wor (100 nmol/L). Wor has been shown to diminish spontaneous autophagy (the upper two of the 2nd vertical row) and reduced stimulus-triggered autophagy (the 3rd vertical row). (D) Flow cytometry assay showing the effect of ZNF32-KD on cell death after a 24 h treatment with H2O2 or diamide with a 3 h pre-treatment with or without Wor (100 nmol/L). (E) The bar results are shown. (F) Atg5 interference efficiency and LC3II expression were detected using western blot analysis. β-actin was used as a loading control. The full size blots were shown in the Supplementary Figure S6 and band of interest is indicated with an arrow. (G) MTT assay showing the effect of si-Atg5 on MCF-7 cell viability after a 24 h treatment with H2O2 or diamide.
