Figure 6
Osteogenic differentiation of BMSCs on the positively-charged surface with tertiary amines in the presence of the NOS inhibitor or siRNA-iNOS transfection.
Expressions of osteogenic markers (Runx-2, ALP, OCN and BSP) of BMSCs cultured in the presence and absence of the NOS inhibitor or siRNA-iNOS transfection in the cell culture medium for 3 days are measured by real time PCR relative to GAPDH expression and normalized to the expressions on Blank without the NOS inhibitor and siRNA. (a) eNOS inhibitor (L-NAME). (b) nNOS inhibitor (Sper). (c) iNOS inhibitor (L-Can). (d) siRNA-iNOS transfection. The bottom left illustrates transfection of siRNA-iNOS with GFP gene into BMSCs and microscopic images of the cells before and after siRNA transfection. (*) and (**) denote two statistical significance (p < 0.05 and p < 0.01), respectively, compared to the sample without NOS inhibitors and siRNA.
