Figure 3
From: Mechanical interplay between invadopodia and the nucleus in cultured cancer cells
The spatial relationships between microtubules and the core actin bundle of invadopodia.
A375 cells, cultured on gelatin and triple labeled for actin (A and C (red)), α-tubulin (B and C (green)) and lamin A/C (blue in Panel C). Z-stacks (0.2 μm apart) of the three colors were acquired, deconvoluted and 3D reconstruction images were generated (C). Top-down and tilted views of the 3D reconstruction and rendering are shown in (D) and (E), respectively. In (F), the 3D reconstruction image (as in D) is superimposed on the triple-labeled image.
