Figure 7
From: Mechanical interplay between invadopodia and the nucleus in cultured cancer cells
LINC complex inhibition increases invadopodia adhesion to the matrix.
(a) A375 cells were cultured on gelatin-coated glass-bottomed dishes for 3 h, than fixed and stained for actin, lamin A/C and SUN1. From left to right: Actin staining (invadopodia structures denoted by white arrows), SUN1 staining in the nuclear lamina and SUN1 TIRF and lamin A/C TIRF microscopy, showing the nuclear indentations. (b) A375 cells were transfected with non-targeting siRNA (siNT), siNESPRIN-2, or siSUN1 and cultured for 3 h on fluorescently labeled gelatin-coated glass-bottomed dishes. Cells were stained for actin, vinculin and DAPI. White arrows denote invadopodia structures, their respective adhesion vinculin ring and matrix degradation. On the right, an overlay of actin (a, red), vinculin (v, green) and DAPI (D, blue). (c) Magnification of the triple overlay (as in b) 1 = siNT, 2 = siNESPRIN-2, 3 = siSUN1. (d) Quantification of the cells that form adhesion rings in siNT (n = 141), siNESPRIN-2 (n = 131) and siSUN1 (n = 155) transfected cells. The results are representative of findings in three repeating experiments. (e) Quantification of the cells that form invadopodia in siNT, siNESPRIN-2 and siSUN1 transfected cells. The results are representative of findings in three repeating experiments.
