Figure 3
Total number of action potentials versus final functional AChR phenotype.
(A) The activity triggered in response to conditioned media was different for all three phenotypes of isolated LPeD1 neurons. The figure shows 10 minutes samples of activity observed at various time points following CM exposure. Three different LPeD1 phenotypes were deciphered by neuronal responses to exogenously applied acetylcholine, which elicited 3 different effects, excitatory (Bi), inhibitory (Bii) and biphasic (Biii; elements of both excitation and inhibition). In (Bi), application of acetylcholine excited the cell, eliciting action potentials and increasing the frequency of activity. In (Bii), acetylcholine inhibited the cell, stopping the firing of action potentials and induced a decrease in neuronal membrane potential. In (Biii), acetylcholine elicited an action potential that was followed by a subsequent decrease in membrane potential (a pre-application potential line is shown as a red dotted line to aid in visualization). Acetylcholine applications are indicated by a solid arrow. The total LPeD1 activity is stratified according to neuronal response to acetylcholine application (C). It can be seen that cells with excitatory response had higher levels of activity than those that exhibited an inhibitory response. Significance is denoted with an asterisk (*) and is assumed if p < 0.05.
