Figure 13

Representative image of A11 dot blot showing effect of compounds 1, 3, 6 and 15 on WtAS A11 epitope formation.

(a) WtAS (210 µM) aggregated alone or co-incubated with equimolar concentration of compound 1, 3, 6 and 15 for 30 days (b) WtAS (210 µM) samples aggregated alone for 60 days or with equimolar concentrations of compounds 1, 3, 6 and 15 added at day 30 and further co-incubated for 30 days. For dot blot analysis 3 μL of WtAS samples were spotted on a nitrocellulose membrane and probed with A11 antibody.