Figure 4

H&E staining and matched FISH analysis of TCV spheroid sections at different time points.

(a) TCV spheroid sections after 9 days, (b) 25 days and (c) 50 days of culture. Red arrows shows cell of maternal origin. Majority of the cells migrating out of the spheroid edges were of fetal origin (b, c). (d) Graph showing the change in the percentage of maternal origin cells in the spheroids at different time points (in days, X axis). The data is represented as mean ± standard deviation (n = 3). Note the significant (p < 0.05) increase in the percentage of maternal cells from day 0 to day 25 of culture. Lower magnification images of the spheroid sections are shown in Supplementary Figure S8. Scale bars, 20 μm.