Figure 3
From: Identification of enzymes involved in SUMOylation in Trypanosoma brucei
Identification of the enzyme activity of TbUba2. (a) In vitro SUMOylation of TbUba2 with or without ATP. The reaction was stopped with 2 × SDS-loading buffer. The samples were fractionated by SDS-PAGE (12%) and analyzed by immune blotting using anti-His antibody. The arrows indicate TbSUMO-His, TbAos1-His, TbUba2-His and the SUMOylated TbUba2-His, respectively. (b) In vitro SUMOylation of TbUbc9-HA in the presence or absence of various components. The samples were fractionated by SDS-PAGE (12%) and analyzed by immune blotting using anti-HA antibody. The arrows indicate TbUbc9-HA and SUMOylated TbUbc9-HA, respectively.
